Preparative Chromatography
Preparative Chromatography For Cannabinoid Separation in Hemp Extract
extraktLAB’s pure99 HPLC chromatography system is an automated, high-pressure liquid chromatography machine for the separation and purification of botanicals and active pharmaceutical ingredients. The chromatography machine is used to separate cannabinoids in the complex botanical matrix.
KEY APPLICATIONS
- Separation of Minor Cannabinoids
- Separation of Nutraceutical or Pharmaceutical API from Botanical Extract
- THC/CBD Remediation
- THC Isolation
Advanced Chromatography For Industrial Hemp and Cannabis Extraction
CBD
Isolation
CBD isolation is a key process in hemp remediation. Do you know how to separate THC from CBD?
Types of Chromatography
There are varying types of chromatography used throughout a number of industries.
Mobile Phase vs Stationary Phase
To understand chromatography, you need to understand to the two basic functions of chromatography.
Flash
Chromatography
Capable of identifying and separating various components efficiently, flash chromatography is essential.
Advanced Chromatography for Botanical Separations
We have created the premier chromatography machine and method for high performance cannabinoid separations from hemp extract and the best CBD chromatography machine for separation of cannabinoids for engineered and tailored formulations.
Why pure99 for Chromatography?
- High recovery depending on cannabinoid ratios.
- Configurable production per hour (be sure to watch our video and read FAQs on production rate)
- Built in solvent recovery system enables solvent recycling on board.
- Fully automated chromatography methods enable stack injections for higher throughput.
- 21 CFR Part 11 data integrity compliance.
- Selectivity for separation of cannabinoids.
- Stable stationary phases that are low-cost.
- High pressure injection.
- GMP compliant and engineering standard compliant including C1D2 compliant, Atex II compliant, PED, ASME Section VIII DIV 2.
Publications on igwLAB
Water-Free Separations
One of the key issues associated with liquid-liquid extraction (CPC, CCC, LLE) is the use of water and alcohol as one of the polar phases. The issue with this is water is very tedious to remove from the end product being extracted. Because water has a very high boiling point and also promotes the production of an emulsified product, water is to be avoided at all costs. Our proprietary methods enable the chromatography paper to completely avoid water in the separation thereby saving time, money, and avoiding a whole lot of hassle.
High Production Output
The throughput of our chromatography systems depends on the column. Our largest column is 13L and will produce multiple kg of broad spectrum hemp extract per hour. The production rate depends on many different factors and your column, your method, and your sample will determine the ultimate throughput. And the throughput will vary depending on your goals. The great thing about the pure99 is that production rate also includes the solvent recovery time for the eluent that is recoverable by the chromatography system. Any solvent left in the sample will need to be recovered separately.
Packable Chromatography Columns
The chromatography columns we sell can be packed by the customer in less than 10 minutes. The packing material itself is a low-cost compared other proprietary packing materials such as molecularly imprinted polymeric materials.
Unlike polymeric packing materials, our stationary phase is also mechanically stable so that the column can be run at high pressure.
Chromatography Methods, Training, SOPs Included
extraktLAB’s pure99 chromatography equipment comes with built in methods selected for the separation of cannabinoids. As a user and researcher, you will decide how pure your extract will be and you will also decide on the recovery that you wish to obtain with this chromatography equipment. These selections and components will impact the overall throughput. You will also decide how many shifts you will have.
Stationary phase is low cost and can be packed by the customer. The material is also highly mechanically stable so the column won’t degrade or collapse at high pressure during the chromatography process.
Integrated Solvent Recycling
As with any chromatography technique that uses solvents, a huge barrier to adoption and use is dealing with all of the solvent generated as the technique is scaled up. One of the key benefits of the pure99 chromatography system is that it automatically recycles and recovers the solvent for reuse.
Solvent loss can be a key contributor to high operating costs if the solvent becomes entrained with the product during the chromatography process, however, this is not the case with the pure99. The pure99 chromatography system incorporates a falling film solvent recovery system, to avoid the hassle of having to do an extremely large amount of solvent recovery.
High Yield
As with any chromatography method, there are always trade-offs between throughput, purity, and selectivity. Preparative chromatography yields a very high recovery rate compared other competing chromatography techniques. For example, it is not uncommon to recover up to 99% of the target cannabinoid. Liquid-liquid techniques typically have much lower recoveries.
But this can also depend on the peak shape and operational parameters such as where the chromatographer decides to make the cuts. Recovery largely depends on what is known in chromatography as selectivity. The greater the separation distance between cannabinoids, the greater the selectivity of the chromatography. The separation of cannabinoids through chromatography is never perfect. In other words, recovery can also depend upon how the chromatography operator selects the psychoactive cannabinoid.
High recovery during chromatography depends primarily on the selectivity of separation.
Feature Packed Chromatography for High Performance Chromatography
Equipment Specifications of the pure99-x Chromatography System
- 13.6 cm high pressure liquid separation chromatography column
- Up to 24 cm column length
- High pressure gradient forming system.
- Integrated closed loop solvent falling film recovery system for ease of solvent recovery.
- Flow meter and pulse dampener for precise flow control.
- High pressure pumping system up to 2500 psi.
- Full software control, automation, chromatography methods automation, and run reporting.
- Pre pump eluent conditioner for high vapor pressure solvents.
- Reloadable media column.
Attribute 244240_0a8ec7-69> |
Value 244240_4b03f6-59> |
---|---|
Separation Column Diameter 244240_de6014-10> |
13.6 cm 244240_d039c0-f1> |
Separator Flow Rate Max 244240_c47c00-b7> |
0-8 LPM 244240_2a8cd6-95> |
Column Dynamic Axial Pressure 244240_ae6810-d0> |
2500 max psi 244240_050f5a-d2> |
Eluent Inlet Lines 244240_a2a71f-16> |
2 244240_18ed29-67> |
User Interface 244240_729118-56> |
15″ Touch Screen 244240_e48012-c7> |
Automated Changeover & Sequences 244240_5eb1a7-1d> |
Yes 244240_8030c3-d0> |
Barcode Tracking 244240_4bc6a9-af> |
Yes 244240_d40f96-08> |
UV-Vis Detector Variable Wavelength 244240_bba79a-99> |
210-600 nm 244240_aec5e3-f3> |
Advanced Chromatography System For Separations and R&D
Equipment Specifications of the pure99-z Chromatography System
The pure99-z is the premier column chromatography system for separations, testing and is designed for research and development operations. Preparative chromatography yields a very high recovery rate compared other competing chromatography techniques. For example, it is not uncommon to recover up to 99% of the target cannabinoid. Liquid-liquid techniques typically have much lower recoveries. High recovery during chromatography depends primarily on the selectivity of separation.
- High pressure column
- Large diameter column for high loadability
- Automated Methods & Data Integrity Controls
- Sample loop up to 100mL
- Column is packable with a variety of materials
- UV/VIS detectors
- Closed loop solvent recovery
Column Upgrades for pure99x & pure99z
Manufactured in the USA by United Science
pure99 Stand Alone Columns provide separation flexibility that is required for every operation. The high maximum pressure and the variable column length allow optimization of the plate number and retention time. The unit comes as a complete assembly on casters that interfaces with the pure99 to control packed bed compression and provide eluent flow and sample injection.
All column upgrades can be added to either pure99.
All column upgrades stand alone on casters and the dimensions for all column upgrades are 60.96cm x60.96cm x132cm
244240_a6a49d-d4> |
130 mm ID Column (1.5L) 244240_659ccd-95> |
140 mm ID Column (3L) 244240_66dc07-b5> |
178 mm ID Column (7L) 244240_1caca0-50> |
178 mm ID Column (10L) 244240_f0b9f5-9d> |
203 mm ID Column (13L) 244240_8b9af4-5c> |
Materials 244240_ee201b-54> |
316 Stainless Steel, PTFE 244240_13787b-29> |
316 Stainless Steel, PTFE, Viton 244240_6aaa32-03> |
316 Stainless Steel, PTFE, Viton 244240_0b1e74-06> |
316 Stainless Steel, PTFE, Viton 244240_9bcd0a-d9> |
316 Stainless Steel, PTFE, Viton 244240_66325d-cd> |
Inside Diameter (cm) 244240_eeeff8-7d> |
13 244240_91c7e1-1e> |
140 244240_f209c9-82> |
178 244240_7ff4ac-7f> |
178 244240_370068-8e> |
203 244240_cf401c-f6> |
Max Pressure (bar) 244240_563af6-fe> |
172 244240_81d8e7-9e> |
344 244240_1cdd47-02> |
195 244240_ad8bcb-90> |
195 244240_afb997-f7> |
284 244240_b2652c-11> |
Maximum Column Volume (L) 244240_05e00e-74> |
1.5 244240_6b4461-14> |
3 244240_7ea94c-ea> |
7 244240_1c2b5a-01> |
10 244240_f1a927-2b> |
13 244240_57e3cc-3c> |
Learn More About Our pure99 Chromatography Machines
What is Chromatography?
extraktLAB has been producing chromatography systems, high pressure liquid chromatography, solid phase extraction, and selective separations materials since 2009. The company was one of the first in the cannabis extraction and hemp industries to implement large scale chromatography separations of cannabinoids to produce tailored formulations with targeted cannabinoid ratios.
Chromatography is a separations technique that separates compounds of interest into their individual components. The combined chromatography equipment and technique is ubiquitous and is used to obtain pure compounds in nearly every industry that processes fluids.
There are many different kinds of chromatography including gas chromatography, liquid chromatography, column chromatography and thin layer chromatography. Distinct from liquid-liquid extractions, gas gas or membrane separations, chromatography uses a solid phase or stationary phase and a liquid/gas phase to accomplish the separation.
Chromatography Explained
Why Use Preparative Chromatography?
Preparative chromatography is a technology used to distinguish the various compounds of a mixture and separating them into their pure components.
In the hemp and cannabis extraction industry, chromatography allows manufacturers to distinguish a list of compounds in the hemp plant and create a specifically formulated product that’s both legal and tailored to the marketplace demands.
Chromatography is also effective at identifying and removing unwanted pesticides, and other residual chemicals. These are essential factors in creating a healthy, safe, desirable product for the end user making High-Pressure Liquid Chromatography an essential part of your production.
Scalable Chromatography Solution
The issue of scalability in preparative chromatography goes hand-in-hand with liquid solvent recovery. One of the things which makes traditional flash chromatography a benchtop or lab scale technique is the quantity of liquid that is consumed. This can drive the costs of production to untenable heights.
The pure99 chromatography machine solves this liquid consumption in chromatography for a scalable solution. With integrated closed loop technology, this chromatography equipment is designed to automatically recover excess volatile eluent.
Liquid intake is recycled back to the beginning of the chromatography column via built in inert gas counterflow distillation. The concept of applying a fractional distillation system to chromatography technology enabled the creation of the first scalable chromatography system of its kind in the industry.
Why Use Endless Product Formulation Potential Chromatography?
Preparative chromatography is a technology used to distinguish the various compoundsThe pure99 High-Pressure Liquid Chromatography (HPLC) system is designed to produce broad spectrum oils and purified isolates. This allows producers to keep desired cannabinoids including CBD, CBG, CBN, while removing psychoactive cannabinoids during the chromatography process. of a mixture and separating them into their pure components.
This cannot be accomplished with a normal isolation manufacturing procedure. pure99 chromatography is capable of the very specific target removal system required to perform this kind of specialized product formulation.
Full-Spectrum and Broad Spectrum products have become increasingly valued by consumers in the hemp and botanical extract market, and chromatography is one of the best methods to ensure quality full and broad spectrum products.
Where normally an extraction method would be manipulated to regulate the amount of psychoactive cannabinoids in a final product formulation, pure99 chromatography allows for the producer to selectively manipulate quantities of specific terpenes and cannabinoids for specific indications tailored to the consumer’s desires.